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Enhancement of production and activity of alkaline zinc metalloprotease from Salinivibrio proteolyticus using low intensity direct electric current and zinc nanoparticles
- Borhani, Matia Sadat, Etemadifar, Zahra, Emtiazi, Giti
- Biotechnology letters 2016 v.38 no.9 pp. 1565-1570
- Salinivibrio, bacteria, buffers, casein, electric current, enzymatic reactions, enzyme activity, hydrochloric acid, metalloproteinases, nanoparticles, pH, zinc, zinc sulfate
- OBJECTIVES: To improve the production and activity of an alkaline zinc metalloprotease from Salinivibrio proteolyticus in response to ZnSO₄ (ionic and nanoparticle forms) and low intensity direct electric current (LIDC). RESULTS: A DC of 50 µA for 10 min increased enzyme production from 35 to 53 U ml⁻¹ when applied to the stationary phase bacterial cells. Zn²⁺ improved enzyme production better than zinc nanoparticles (52 vs. 43.5 U ml⁻¹). Zinc nanoparticles (0.5 mM) added to an enzyme reaction mixture containing casein (0.65 %) and 20 mM Tris/HCl buffer (pH 8) improved enzyme activity more than Zn²⁺ (42 vs. 36 U ml⁻¹). CONCLUSION: LIDC exposure (50 µA, 10 min) to the stationary phase bacterial cells increases metalloprotease production in Salinivibrio. A low concentration of zinc nanoparticles (0.5 mM) increases maximum enzyme activity.