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Direct determination of trace refractory elements in human serum by ETV–ICP–MS with in-situ matrix removal

Author:
Li, Shengqing, Hu, Bin, Jiang, Zucheng, Chen, Rui
Source:
Analytical and bioanalytical chemistry 2004 v.379 no.7-8 pp. 1076-1082
ISSN:
1618-2642
Subject:
barium, blood serum, calcium, chlorine, chromium, detection limit, humans, magnesium, molybdenum, potassium, sodium, statistical analysis, sulfur, temperature, vanadium, volatilization
Abstract:
A method for in-situ removal of matrix is proposed for direct determination of trace refractory elements in human serum by ETV–ICP–MS with the use of poly(tetrafluoroethylene) (PTFE) as fluorinating reagent. Attention has been paid to investigating the vaporization behavior both of refractory elements of interest and of matrix elements (Na, K, Ca, Mg, Cl, S, and P) in a graphite furnace with the PTFE modifier present or not. It was shown that potential interferences from the organic and inorganic matrices in the serum sample could be eliminated or reduced to a negligible level by appropriate dilution of the serum and deliberate optimization of the ETV temperature program. The proposed method has been applied to the direct simultaneous determination of V, Cr, Mo, Ba, La, Ce, and W in human serum. The limits of detection for fivefold diluted serum were 0.18 (V), 0.229 (Cr), 0.050 (Mo), 0.328 (Ba), 0.031 (La), 0.038 (Ce), and 0.019 ng mL⁻¹ (W), respectively, and the relative standard deviations of the method were in the range 4–15% (2 ng mL⁻¹ in serum, n=3).
Agid:
5481037