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Effects of nitrogen on the lipid and carotenoid accumulation of oleaginous yeast Sporidiobolus pararoseus

Author:
Han, Mei, Xu, Zhi-yuan, Du, Chao, Qian, He, Zhang, Wei-Guo
Source:
Bioprocess and biosystems engineering 2016 v.39 no.9 pp. 1425-1433
ISSN:
1615-7591
Subject:
Sporidiobolus, antioxidant activity, beta-carotene, carbon, fermentation, free radical scavengers, lipid content, lipid peroxidation, nitrogen, oleic acid, yeasts
Abstract:
Nitrogen limited but carbon excess condition was used to obtain high cellular lipid content and production. The maximum lipid production was 51 g/L, the lipid content in the dry cell was 60 %, and the lipid productivity was 0.53 g/L/h. In the fermentation, the content of lipid was raised from 20 % of dry cell weight to 60 %, and the proportion of oleic acid was raised from 66.8 to 72.5 %. Meanwhile, the metabolism of carotenoids switched to torulene, and its proportion was raised from 30 to 58 %. This was according to torulene had the better antioxidant ability than β-carotene to protect the strain from oxidative damage proved by their ABTS* radical scavenging activity and lipid peroxidation inhibition ability. Sporidiobolus pararoseus lipid was a good source of lipid not only because of its high oleic acid composition, but also the antioxidant ability of carotenoids in the lipid.
Agid:
5481489