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Response surface analysis for the production of an enantioselective lipase from Aspergillus niger by solid-state fermentation
- Contesini, Fabiano Jares, da Silva, Vania Castriani Fernades, Maciel, Rafael Ferreira, de Lima, Rosemary Joana, Barros, Francisco Fábio Cavalcante, de Oliveira Carvalho, Patrícia
- The journal of microbiology 2009 v.47 no.5 pp. 563
- Aspergillus niger, calcium chloride, carboxylic ester hydrolases, culture media, enantiomers, enzyme activity, glucose, magnesium sulfate, manganese sulfate, mathematical models, model validation, oleic acid, olive oil, peptones, potassium dihydrogen phosphate, response surface methodology, screening, sodium chloride, sodium phosphate, solid state fermentation, temperature, water content, yeast extract
- The lipase produced by the Aspergillus niger strain AC-54 has been widely studied due to its enantioselectivity for racemic mixtures. This study aimed to optimize the production of this enzyme using statistical methodology. Initially a Plackett-Burman (PB) design was used to evaluate the effects of the culture medium components and the culture conditions. Twelve factors were screened: water content, glucose, yeast extract, peptone, olive oil, temperature, NaH₂P0₄, KH₂P0₄, MgS0₄-7H₂0, CaCl₂, NaCI, and MnS0₄. The screening showed that the significant factors were water content, glucose, yeast extract, peptone, NaH₂P0₄, and KH₂P0₄, which were optimized using response surface methodology (RSM) and a mathematical model obtained to explain the behavioral process. The best lipase activity was attained using the following conditions: water content (20%), glucose (4.8%), yeast extract (4.0%), and NaH2P04 (4.0%). The predicted lipase activity was 33.03 U/ml and the experimental data confirmed the validity of the model. The enzymatic activity was expressed as µmoles of oleic acid released per minute of reaction (µmol/min).