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Cloning of genes related to aliphatic glucosinolate metabolism and the mechanism of sulforaphane accumulation in broccoli sprouts under jasmonic acid treatment

Author:
Guo, Liping, Yang, Runqiang, Gu, Zhenxin
Source:
Journal of the science of food and agriculture 2016 v.96 no.13 pp. 4329-4336
ISSN:
0022-5142
Subject:
acid treatment, biochemical pathways, biosynthesis, broccoli, complementary DNA, cytochrome P-450, gene expression, glucoraphanin, jasmonic acid, molecular cloning, thioglucosidase, unspecific monooxygenase
Abstract:
BACKGROUND: Cytochrome P450 79F1 (CYP79F1), cytochrome P450 83A1 (CYP83A1), UDP‐glucosyltransferase 74B1 (UGT74B1), sulfotransferase 18 (ST5b) and flavin‐containing monooxygenase GS‐OX1 (FMOGS ‐ OX₁) are important enzymes in aliphatic glucosinolate biosynthesis. In this study, their full‐length cDNA in broccoli was firstly cloned, then the mechanism of sulforaphane accumulation under jasmonic acid (JA) treatment was investigated. RESULTS: The full‐length cDNA of CYP79F1, CYP83A1, UGT74B1, ST5b and FMOGS ‐ OX₁ comprised 1980, 1652, 1592, 1378 and 1623 bp respectively. The increase in aliphatic glucosinolate accumulation in broccoli sprouts treated with JA was associated with elevated expression of genes in the aliphatic glucosinolate biosynthetic pathway. Application of 100 µmol L⁻¹ JA increased myrosinase (MYR) activity but did not affect epithiospecifier protein (ESP) activity in broccoli sprouts, which was supported by the expression of MYR and ESP. Sulforaphane formation in 7‐day‐old sprouts treated with 100 µmol L⁻¹ JA was 3.36 and 1.30 times that in the control and 300 µmol L⁻¹ JA treatment respectively. CONCLUSION: JA enhanced the accumulation of aliphatic glucosinolates in broccoli sprouts via up‐regulation of related gene expression. Broccoli sprouts treated with 100 µmol L⁻¹ JA showed higher sulforphane formation than those treated with 300 µmol L⁻¹ JA owing to the higher glucoraphanin content and myrosinase activity under 100 µmol L⁻¹ JA treatment. © 2016 Society of Chemical Industry
Agid:
5483385