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Eukaryotic elongation factor 1-beta interacts with the 5′ untranslated region of the M gene of Nipah virus to promote mRNA translation

Author:
Uchida, Shotaro, Sato, Hiroki, Yoneda, Misako, Kai, Chieko
Source:
Archives of virology 2016 v.161 no.9 pp. 2361-2368
ISSN:
0304-8608
Subject:
Nipah virus, gene expression regulation, messenger RNA, nucleotides, reporter genes, translation (genetics)
Abstract:
Nipah virus belongs to the genus Henipavirus in the family Paramyxoviridae, and its RNA genome is larger than those of other paramyxoviruses because it has long untranslated regions (UTRs) in each gene. However, the functions of these UTRs are not fully understood. In this study, we investigated the functions of the 5′ UTRs and found that the 5′ UTR of the M gene upregulated the translation of a reporter gene. Using an RNA pull-down assay, we showed that eukaryotic elongation factor 1-beta (EEF1B2) interacts with nucleotides 81–100 of the M 5′ UTR and specifically enhances its translation efficiency. Our results suggest that the M 5′ UTR promotes the production of M protein and viral budding by recruiting EEF1B2.
Agid:
5483926