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Isolation and characterization of a distinct duck-origin goose parvovirus causing an outbreak of duckling short beak and dwarfism syndrome in China

Author:
Chen, Shilong, Wang, Shao, Cheng, Xiaoxia, Xiao, Shifeng, Zhu, Xiaoli, Lin, Fengqiang, Wu, Nanyang, Wang, Jinxiang, Huang, Meiqing, Zheng, Min, Chen, Shaoying, Yu, Fusong
Source:
Archives of virology 2016 v.161 no.9 pp. 2407-2416
ISSN:
0304-8608
Subject:
Anseriform dependoparvovirus 1, allantoic fluid, antigens, beak, cytopathogenicity, ducklings, dwarfing, etiological agents, fibroblasts, flocks, monoclonal antibodies, morbidity, mortality, tongue, viruses, weight loss, China
Abstract:
Many mule duck and Cherry Valley duck flocks in different duck-producing regions of China have shown signs of an apparently new disease designated “short beak and dwarfism syndrome” (SBDS) since 2015. The disease is characterized by dyspraxia, weight loss, a protruding tongue, and high morbidity and low mortality rates. In order to characterize the etiological agent, a virus designated SBDSV M15 was isolated from allantoic fluid of dead embryos following serial passage in duck embryos. This virus causes a cytopathic effect in duck embryo fibroblast (DEF) cells. Using monoclonal antibody diagnostic assays, the SBDSV M15 isolate was positive for the antigen of goose parvovirus but not Muscovy duck parvovirus. A 348-bp (2604-2951) VP1gene fragment was amplified, and its sequence indicated that the virus was most closely related to a Hungarian GPV strain that was also isolated from mule ducks with SBDS disease. A similar disease was reproduced by inoculating birds with SBDSV M15. Together, these data indicate that SBDSV M15 is a GPV-related parvovirus causing SBDS disease and that it is divergent from classical GPV isolates.
Agid:
5483930