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Phylogenetic characterisation of feline immunodeficiency virus in naturally infected cats in Croatia indicates additional heterogeneity of subtype B in Europe

Perharić, Matko, Biđin, Marina, Starešina, Vilim, Milas, Zoran, Turk, Nenad, Štritof, Zrinka, Hađina, Suzana, Habuš, Josipa, Stevanović, Vladimir, Mojčec-Perko, Vesna, Kovač, Snježana, Martinković, Krešimir, Barbić, Ljubo
Archives of virology 2016 v.161 no.9 pp. 2567-2573
Feline immunodeficiency virus, antibodies, blood sampling, cats, diagnostic techniques, genes, immunoaffinity chromatography, phylogeny, polymerase chain reaction, sequence analysis, Croatia
This study was performed on 29 domestic cats with a variety of clinical signs, possibly related to FIV infection. Blood samples were tested by a rapid immunochromatographic (ICA) procedure for detection of FIV antibodies. Subsequently, polymerase chain reaction (PCR) was performed to amplify a portion of the proviral gag gene. All 11 positive PCR products were sequenced and compared with previously reported FIV sequences. Croatian proviral isolates that could be amplified were clustered within subtype B, and additional heterogeneity was confirmed by the formation of three separate clusters. Phylogenetic analysis of circulating strains in Croatia and in southeast Europe is necessary to improve diagnostic methods and selection of the appropriate vaccinal strains.