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Complete genome sequence of a novel sub-subgenotype 2.1g isolate of classical swine fever virus from China

Author:
Gong, Wenjie, Zhang, Li, Lu, Zongji, Jia, Junjie, Wang, Meng, Peng, Zhicheng, Guo, Huancheng, Shi, Jishu, Tu, Changchun
Source:
Archives of virology 2016 v.161 no.9 pp. 2613-2617
ISSN:
0304-8608
Subject:
3' untranslated regions, 5' untranslated regions, Classical swine fever virus, amino acid sequences, amino acid substitution, genome, kidneys, nucleotides, open reading frames, phylogeny, polyproteins, sequence analysis, topology, virulence, viruses, China
Abstract:
Current subgenotype 2.1 isolates of classical swine fever virus (CSFV) play a dominant role in CSF outbreaks in China, and a novel sub-subgenotype 2.1g of CSFV was recently identified, but the complete genome sequence of this new sub-subgenotype has not been reported. In this study, complete genome of 2.1g isolate GD19/2011 collected from Guangdong province of China in 2011 was sequenced. It was found to be 12,298 nucleotides (nt) in length, including a 375-nt 5’UTR, a 11,697-nt opening reading frame (ORF), and a 227-nt 3’UTR. GD19/2011 shared 91.0-93.7 % and 95.6-97.5 % nt and amino acid sequence identity, respectively, with other subgenotype 2.1 isolates. The topology of a phylogenetic tree constructed based on complete genome sequences of GD19/2011 and other CSFV isolates was identical to that obtained with full-length E2 gene sequences, but it was significantly different from those obtained with the 5’UTR and core sequences. Serial passages of GD9/2011 in PK-15 cells generated a highly cell-adapted virus stock with an infectious titer of 10⁷.⁸ TCID₅₀/ml at the 12ᵗʰ passage in which two amino acid substitutions, S476R and N2494S, were observed in comparison with the complete polyprotein sequence of the original isolate from kidney tissue, GD19/2011. This is the first report of the complete genome sequence of a 2.1g isolate, and the GD19/2011 isolate will be useful for further analysis of the evolution and virulence of CSFV isolates.
Agid:
5483934