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Detection and characterization of a novel genotype of porcine astrovirus 4 from nasal swabs from pigs with acute respiratory disease

Author:
Padmanabhan, Aiswaria, Hause, Ben M.
Source:
Archives of virology 2016 v.161 no.9 pp. 2575-2579
ISSN:
0304-8608
Subject:
Porcine astrovirus, RNA, amino acid sequences, genes, genotype, metagenomics, nose, nucleotide sequences, respiratory tract diseases, sequence analysis, swine, Oklahoma
Abstract:
Metagenomic sequencing of three nasal swabs collected from 10- to 21-day-old pigs exhibiting unexplained acute respiratory disease from two different commercial production facilities in Oklahoma identified a novel genotype of porcine astrovirus 4 (PAstV-4). The genomes had only ~75 % nucleotide sequence identity to previously characterized PAstV-4 isolates, while the capsid-encoding ORF2 had only ~53 % amino acid sequence identity to reference strains. A TaqMan assay targeting the novel ORF2 gene found 21 % and 19 % incidence in nasal and fecal swabs, respectively, submitted for unrelated diagnostic testing. PAstV-4 RNA levels were significantly higher (P = 0.04) in nasal swabs, suggesting a possible atypical respiratory tropism.
Agid:
5483935