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Molecular characterization of a novel putative ampelovirus tentatively named grapevine leafroll-associated virus 13
- Ito, Takao, Nakaune, Ryoji
- Archives of virology 2016 v.161 no.9 pp. 2555-2559
- Ampelovirus, Grapevine leafroll-associated virus, RNA-directed RNA polymerase, Vitis, coat proteins, genome, heat-shock protein 70, open reading frames, phylogeny, viruses
- A novel putative ampelovirus was detected in grapevines that showed typical leafroll symptoms and was tentatively named grapevine leafroll-associated virus (GLRaV)-13 following the series of numbering of other GLRaVs. The complete genome of GLRaV-13 comprised 17,608 nt and contained eleven putative open reading frames, showing genetic features similar to those of viruses belonging to subgroup I of genus Ampelovirus. Phylogenetic trees based on the RNA-dependent RNA polymerase, heat shock protein 70 homolog, and coat protein showed that GLRaV-13 had the closest, but still distant, relationship to GLRaV-1 in the subgroup I cluster.