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Acacia honey accelerates in vitro corneal ulcer wound healing model
- Abd Ghafar, Norzana, Ker-Woon, Choy, Hui, Chua Kien, Mohd Yusof, Yasmin Anum, Wan Ngah, Wan Zurinah
- BMC complementary and alternative medicine 2016 v.16 no.1 pp. 259
- Acacia, New Zealand White rabbit, actin, aldehyde dehydrogenase, alternative medicine, blood serum, collagen, cornea, fibroblasts, gene expression, genes, honey, immunocytochemistry, metalloproteinases, models, muscles, protein synthesis, quantitative polymerase chain reaction, rabbits, reverse transcriptase polymerase chain reaction, tissue repair, vimentin
- BACKGROUND: The study aimed to evaluate the effects of Acacia honey (AH) on the migration, differentiation and healing properties of the cultured rabbit corneal fibroblasts. METHODS: Stromal derived corneal fibroblasts from New Zealand White rabbit (n = 6) were isolated and cultured until passage 1. In vitro corneal ulcer was created using a 4 mm corneal trephine onto confluent cultures and treated with basal medium (FD), medium containing serum (FDS), with and without 0.025 % AH. Wound areas were recorded at day 0, 3 and 6 post wound creation. Genes and proteins associated with wound healing and differentiation such as aldehyde dehydrogenase (ALDH), vimentin, alpha-smooth muscle actin (α-SMA), collagen type I, lumican and matrix metalloproteinase 12 (MMP12) were evaluated using qRT-PCR and immunocytochemistry respectively. RESULTS: Cells cultured with AH-enriched FDS media achieved complete wound closure at day 6 post wound creation. The cells cultured in AH-enriched FDS media increased the expression of vimentin, collagen type I and lumican genes and decreased the ALDH, α-SMA and MMP12 gene expressions. Protein expression of ALDH, vimentin and α-SMA were in accordance with the gene expression analyses. CONCLUSION: These results demonstrated AH accelerate corneal fibroblasts migration and differentiation of the in vitro corneal ulcer model while increasing the genes and proteins associated with stromal wound healing.