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Engineering broad-spectrum digestion of polyuronides from an exolytic polysaccharide lyase

Author:
MacDonald, Logan C., Weiler, Elizabeth B., Berger, Bryan W.
Source:
Biotechnology for biofuels 2016 v.9 no.1 pp. 43
ISSN:
1754-6834
Subject:
Chlorophyta, Phaeophyceae, Stenotrophomonas maltophilia, alginate lyase, bioethanol, chemical degradation, digestion, energy efficiency, engineering, macroalgae, mutants, polyuronides, saccharification
Abstract:
BACKGROUND: Macroalgae represents a promising source of fermentable carbohydrates for use in the production of energy efficient biofuel. The primary carbohydrate in brown algae is the uronic acid-containing alginate, whereas green algae contains a significant amount of glucuronan. A necessary step in the conversion of these polyuronides to bioethanol is saccharification, which can be achieved by enzymatic or chemical degradation. RESULTS: Polysaccharide lyases are a class of enzymes which cleave uronic acid-containing glycans via a β-elimination mechanism, acting both endo- and exolytically on their substrates. In the present work, we characterize a putative alginate lyase from Stenotrophomonas maltophilia K279a (Smlt2602) and describe a H208F mutant that, in addition to cleaving alginate-based substrates, displays significant, exolytic glucuronan activity. CONCLUSIONS: To our knowledge this is the first polysaccharide lyase to act exolytically on glucuronan and is an attractive candidate for the broad-spectrum digestion of polyuronides into fermentable monomers.
Agid:
5486612