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Separation of three anthraquinone glycosides including two isomers by preparative high‐performance liquid chromatography and high‐speed countercurrent chromatography from Rheum tanguticum Maxim. ex Balf
- Chen, Tao, Li, Hongmei, Zou, Denglang, Liu, Yongling, Chen, Chen, Zhou, Guoying, Li, Yulin
- Journal of separation science 2016 v.39 no.16 pp. 3105-3112
- Rheum tanguticum, anthraquinones, antioxidant activity, countercurrent chromatography, cytotoxicity, glycosides, high performance liquid chromatography, isomers, medicinal properties
- Anthraquinone glycosides, such as chrysophanol 1‐O‐β‐d‐glucoside, chrysophanol 8‐O‐β‐d‐glucoside, and physion 8‐O‐β‐d‐glucoside, are the accepted important active components of Rheum tanguticum Maxim. ex Balf. due to their pharmacological properties: antifungal, antimicrobial, cytotoxic, and antioxidant activities. However, an effective method for the separation of the above‐mentioned anthraquinone glycosides from this herb is not currently available. Especially, greater difficulty existed in the separation of the two isomers chrysophanol 1‐O‐β‐d‐glucoside and chrysophanol 8‐O‐β‐d‐glucoside. This study demonstrated an efficient strategy based on preparative high‐performance liquid chromatography and high‐speed countercurrent chromatography for the separation of the above‐mentioned anthraquinone glycosides from Rheum tanguticum Maxim.ex Balf.