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Novel Secretory Protein Ss-Caf1 of the Plant-Pathogenic Fungus Sclerotinia sclerotiorum Is Required for Host Penetration and Normal Sclerotial Development

Author:
Xiao, Xueqiong, Xie, Jiatao, Cheng, Jiasen, Li, Guoqing, Yi, Xianhong, Jiang, Daohong, Fu, Yanping
Source:
Molecular plant-microbe interactions 2014 v.27 no.1 pp. 40-55
ISSN:
0894-0282
Subject:
EF hand motifs, Nicotiana benthamiana, Sclerotinia sclerotiorum, appressoria, cell death, genes, leaves, mutants, oxalic acid, pathogenicity, phenotype, plant pathogenic fungi, proteins, rapeseed, sclerotia, signal peptide, tissues, tobacco, transfer DNA
Abstract:
To decipher the mechanism of pathogenicity in Sclerotinia sclerotiorum, a pathogenicity-defective mutant, Sunf-MT6, was isolated from a T-DNA insertional library. Sunf-MT6 could not form compound appressorium and failed to induce lesions on leaves of rapeseed though it could produce more oxalic acid than the wild-type strain. However, it could enter into host tissues via wounds and cause typical necrotic lesions. Furthermore, Sunf-MT6 produced fewer but larger sclerotia than the wild-type strain Sunf-M. A gene, named Ss-caf1, was disrupted by T-DNA insertion in Sunf-MT6. Gene complementation and knockdown experiments confirmed that the disruption of Ss-caf1 was responsible for the phenotypic changes of Sunf-MT6. Ss-caf1 encodes a secretory protein with a putative Ca²⁺-binding EF-hand motif. High expression levels of Ss-caf1 were observed at an early stage of compound appressorium formation and in immature sclerotia. Expression of Ss-caf1 without signal peptides in Nicotiana benthamiana via Tobacco rattle virus-based vectors elicited cell death. These results suggest that Ss-caf1 plays an important role in compound appressorium formation and sclerotial development of S. sclerotiorum. In addition, Ss-Caf1 has the potential to interact with certain host proteins or unknown substances in host cells, resulting in subsequent host cell death.
Agid:
5488833