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An environmental DNA assay for detecting Arctic grayling in the upper Missouri River basin, North America

Author:
Carim, K. J., Dysthe, J. C. S., Young, M. K., McKelvey, K. S., Schwartz, M. K.
Source:
Conservation genetics resources 2016 v.8 no.3 pp. 197-199
ISSN:
1877-7252
Subject:
DNA, Thymallus arcticus, nontarget organisms, quantitative polymerase chain reaction, sympatry, watersheds, Missouri River, Northwestern United States
Abstract:
The upper Missouri River basin in the northwestern US contains disjunct Arctic grayling (Thymallus arcticus) populations of conservation concern. To assist efforts aimed at understanding Artic grayling distribution, we developed a quantitative PCR assay to detect the presence of Arctic grayling DNA in environmental samples. The assay amplified low concentrations of Arctic grayling DNA consistently, and did not amplify non-target species, including sympatric salmonid fishes.
Agid:
5489620