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Great genetic diversity of rotaviruses detected in piglets with diarrhea in Thailand

Author:
Yodmeeklin, Arpaporn, Khamrin, Pattara, Chuchaona, Watchaporn, Saikruang, Wilaiporn, Kongkaew, Aphisek, Vachirachewin, Ratchaya, Kumthip, Kattareeya, Okitsu, Shoko, Ushijima, Hiroshi, Maneekarn, Niwat
Source:
Archives of virology 2016 v.161 no.10 pp. 2843-2849
ISSN:
0304-8608
Subject:
Rotavirus A, diarrhea, feces, genetic variation, genotype, piglets, reverse transcriptase polymerase chain reaction, Thailand
Abstract:
A total of 491 fecal specimens collected from diarrheic piglets in Thailand from January 2011 to March 2014 were screened for group A rotavirus by RT-PCR assay. The G and P genotypes of the detected rotaviruses were determined by multiplex PCR or nucleotide sequencing. Group A rotaviruses were detected in 113 out of 491 (23.0 %) fecal specimens. A wide variety of G-P genotype combinations were identified, and G4P[13] was the most prevalent genotype combination (29.2 %), followed by G4P[23] (14.1 %), G5P[23] (11.5 %), G4P[6] (9.7 %), G3P[23] (7.0 %), G5P[13] (6.1 %), G3P[13] (4.4 %), G3P[6] (2.7 %), and G5P[6] (2.7 %). In addition, the other G-P combinations were also detected at a low percentage, including G3P[19], G4P[7], G9P[19], G9P[23], G9P[7], G4P[19], and G11P[13] strains. This study indicated that group A rotaviruses are a common causes of diarrhea in piglets and a great diversity of G and P genotype combinations are circulating in piglets in Thailand.
Agid:
5491088