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Effects of potential probiotic Lactococcus lactis subsp. lactis on digestive enzymatic activities of live feed Artemia franciscana

Loh, Jiun Yan, Ting, Adeline Su Yien
Aquaculture international 2016 v.24 no.5 pp. 1341-1351
Artemia franciscana, Lactococcus lactis subsp. lactis, beta-glucosidase, bioencapsulation, carboxylic ester hydrolases, digestive enzymes, endo-1,4-beta-glucanase, enzyme activity, fish, larvae, live feeds, probiotics, proteinases, shrimp
In the present study, cellulase, protease, lipase and amylase activities were performed to investigate the effects of Lactococcus lactis subsp. lactis CF4MRS bioencapsulation of Artemia franciscana. Our results show that cellulase activities (total cellulase—FPase activity, exoglucanase and endoglucanase—CMCase activity) were significantly higher (P < 0.05) in A. franciscana tissue homogenates compared to those in the control group after 8 h of L. lactic bioencapsulation. Notably, an exception case was found in β-D-glucosidase activity, whereby the cellulase activity was not significantly different (P > 0.05) compared to the control group. Administrations of L. lactis at cell concentration of 10⁸ CFU mL⁻¹ showed considerable improvement of other important enzymatic activities such as amylase, protease and lipase in A. franciscana. The amylase/protease ratio in probiotic-treated A. franciscana was recorded at 0.343, approximately two times higher than those without probiotic administration (0.184). In contrary, amylase/lipase ratio showed half of a reduction (0.330) in L. lactis-administrated A. franciscana compared to the control (0.614). Our study suggests that important digestive enzymes, e.g., cellulase, amylase, protease and lipase, can be enhanced through bioencapsulation of A. franciscana with L. lactis subsp. lactis, which could in turn lead to further stimulation of endogenous enzymes in the fish and shrimp larvae.