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Chemiluminescent Enzyme Immunoassay for Rapid Detection of Three α-Cyano Pyrethroid Residues in Agricultural Products

Taheri, Niusha, Lan, Meijing, Wei, Peng, Liu, Rui, Gui, Wenjun, Guo, Yirong, Zhu, Guonian
Food analytical methods 2016 v.9 no.10 pp. 2896-2905
chemiluminescence, cowpeas, cross reaction, cypermethrin, deltamethrin, eggplants, enzyme immunoassays, fenpropathrin, gas chromatography, inhibitory concentration 50, lambda-cyhalothrin, monoclonal antibodies, pyrethrins, rapid methods
An indirect competitive chemiluminescent enzyme immunoassay (CLEIA) using a new broad-specific monoclonal antibody was developed for the determination of α-cyano pyrethroids. Under optimum conditions, this assay exhibited high sensitivities toward fenpropathrin, deltamethrin, and λ-cyhalothrin, with a wide detection range of 0.16 to 100 ng/mL and half-maximal inhibition concentration (IC₅₀) of 1.9, 3.4, and 4.3 ng/mL, respectively. Cross-reactivity with other pyrethroids was not detected, except for cypermethrin with 5 %. The accuracy and repeatability of the established CLEIA were evaluated by intra-day and inter-day tests of spiked recovery. For agricultural samples such as orange, eggplant, and cowpea, the average recoveries ranged from 79.6 to 115.5 %, and the intra-day and inter-day coefficients of variation were from 4.7 to 12.2 % and 6.7 to 14.2 %, respectively. Furthermore, analysis of several real samples by the CLEIA was validated by gas chromatography equipped with an electron capture detector, showing good correlation between the two methods. Therefore, the CLEIA presented in this study is suitable as a rapid screening tool for the detection of three α-cyano pyrethroids in agricultural products.