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Fractionation of Plant Bioactives from Black Carrots (Daucus carota subspecies sativus varietas atrorubens Alef.) by Adsorptive Membrane Chromatography and Analysis of Their Potential Anti-Diabetic Activity

Esatbeyoglu, Tuba, Rodríguez-Werner, Miriam, Schlösser, Anke, Liehr, Martin, Ipharraguerre, Ignacio, Winterhalter, Peter, Rimbach, Gerald
Journal of agricultural and food chemistry 2016 v.64 no.29 pp. 5901-5908
Daucus carota, alpha-amylase, alpha-glucosidase, anthocyanins, carrots, chlorogenic acid, chromatography, color, dipeptidyl-peptidase IV, dose response, food coloring, fractionation, glucagon-like peptide 1, glucose, glycemic effect, human cell lines, phenolic acids, polyphenols, secretion
Black and purple carrots have attracted interest as colored extracts for coloring food due to their high content of anthocyanins. This study aimed to investigate the polyphenol composition of black carrots. Particularly, the identification and quantification of phenolic compounds of the variety Deep Purple carrot (DPC), which presents a very dark color, was performed by HPLC-PDA and HPLC-ESI-MSⁿ analyses. The separation of polyphenols from a DPC XAD-7 extract into an anthocyanin fraction (AF) and co-pigment fraction (CF; primarily phenolic acids) was carried out by membrane chromatography. Furthermore, possible anti-diabetic effects of the DPC XAD-7 extract and its AF and CF were determined. DPC samples (XAD-7, CF, and AF) inhibited α-amylase and α-glucosidase in a dose-dependent manner. Moreover, DPC XAD-7 and chlorogenic acid, but not DPC CF and DPC AF, caused a moderate inhibition of intestinal glucose uptake in Caco-2 cells. However, DPC samples did not affect glucagon-like peptide-1 (GLP-1) secretion and dipeptidyl peptidase IV (DPP-4) activity. Overall, DPC exhibits an inhibitory effect on α-amylase and α-glucosidase activity and on cellular glucose uptake indicating potential anti-diabetic properties.