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In vitro activity of plant extracts against biofilm-producing food-related bacteria

Author:
Nostro, Antonia, Guerrini, Alessandra, Marino, Andreana, Tacchini, Massimo, Di Giulio, Mara, Grandini, Alessandro, Akin, Methap, Cellini, Luigina, Bisignano, Giuseppe, Saraçoğlu, Hatice T.
Source:
International journal of food microbiology 2016 v.238 pp. 33-39
ISSN:
0168-1605
Subject:
Escherichia coli, Ficus carica, Juglans regia, Listeria monocytogenes, Olea europaea, Pseudomonas aeruginosa, Punica granatum, Rhus coriaria, Staphylococcus aureus, anti-infective agents, antibacterial properties, antioxidant activity, antioxidants, apigenin, bacteria, biofilm, high performance liquid chromatography, leaves, methanol, minimum inhibitory concentration, plant extracts, polyphenols, quercitrin
Abstract:
The identification of effective antimicrobial agents also active on biofilms is a topic of crucial importance in food and industrial environment. For that purpose methanol extracts of Turkish plants, Ficus carica L., Juglans regia L., Olea europaea L., Punica granatum L. and Rhus coriaria L., were investigated. Among the extracts, P. granatum L. and R. coriaria L. showed the best antibacterial activity with minimum inhibitory concentrations (MIC) of 78–625μg/ml for Listeria monocytogenes and Staphylococcus aureus and 312–1250μg/ml for Escherichia coli and Pseudomonas aeruginosa. SubMICs produced a significant biofilm inhibition equal to 80–60% for L. monocytogenes and 90–80% for S. aureus. The extracts showed also the highest polyphenol content and the strongest antioxidant activity. Bioassay-guided and HPLC procedures demonstrated the presence of apigenin 4′-O-β-glucoside in P. granatum L. and myricetrin and quercitrin in R. coriaria L. Antigenotoxicity of plant extracts was also observed The present findings promote the value-adding of P. granatum L. and R. coriaria L. leaves as natural antimicrobial/antioxidant agents for control of food-related bacterial biofilms.
Agid:
5495828