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Simultaneous saccharification and fermentation of hemicellulose to butanol by a non-sporulating Clostridium species

Author:
Li, Tinggang, He, Jianzhong
Source:
Bioresource technology 2016 v.219 pp. 430-438
ISSN:
0960-8524
Subject:
Clostridium, acetone, arabinose, biofuels, biomass, butanol, carbon, cost effectiveness, enzymes, ethanol, fermentation, genes, glucose, lignocellulose, microorganisms, saccharification, xylan, xylose
Abstract:
Production of lignocellulosic butanol has drawn increasing attention. However, currently few microorganisms can produce biofuels, particularly butanol, from lignocellulosic biomass via simultaneous saccharification and fermentation. Here we report discovery of a wild-type, mesophilic Clostridium sp. strain MF28 that ferments xylan to produce butanol (up to 3.2g/L) without the addition of saccharolytic enzymes and without any chemical pretreatments. Application of selective pressure from 2-deoxy-d-glucose facilitated isolation of strain MF28, which exhibits inactivation of genes (gid and ccp genes) responsible for carbon catabolite repression, thus allowing strain MF28 to simultaneously ferment a combination of glucose (30g/L), xylose (15g/L), and arabinose (15g/L) to produce 11.9g/L of butanol. Strain MF28 possesses several unique features: (i) non-sporulating, (ii) no acetone/ethanol, (iii) complete hemicellulose-binding enzymatic domain, and (iv) absence of carbon catabolite repression. These unique characteristics demonstrate the industrial potential of strain MF28 for cost-effective biofuel generation from lignocellulosic biomass.
Agid:
5496693