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Bacterial communities and enzymatic activities in the vegetation-activated sludge process (V-ASP) and related advantages by comparison with conventional constructed wetland

Yuan, Jiajia, Dong, Wenyi, Sun, Feiyun, Zhao, Ke, Du, Changhang, Shao, Yunxian
Bioresource technology 2016 v.220 pp. 341-351
Proteobacteria, activated sludge, bacterial communities, biomass, community structure, constructed wetlands, enzyme activity, high-throughput nucleotide sequencing, microbial activity, nitrogen, nitrogen-fixing bacteria, phosphorus, pollutants, sewage treatment, temperature, vegetation
A new-developed vegetation-activated sludge process (V-ASP) was implemented for decentralized domestic wastewater treatment, and studied in lab-scale and full-scale. The main purpose of this work was the investigation of biomass activities and microbial communities in V-ASP by comparison with conventional constructed wetland (CW), to unveil the causations of its consistently higher pollutants removal efficiencies. Compared with CWs, V-ASP has greater vegetation nitrogen and phosphorus uptake rates, higher biomass and enzymatic activities, and more bacteria community diversity. The microbial community structure was comprehensively analyzed by using high-throughput sequencing. It was observed that Proteobacteria was dominated in both CWs and V-ASPs, while their subdivisions distribution was rather different. V-ASPs contained a higher nitrite-oxidizing bacteria (Nitrospira) abundances that resulted in a consistently better nitrogen removal efficiency. Hence, a long-term experiment of full-scale V-ASP displayed stably excellent capability in resistance of influent loading shocks and seasonal temperature effect.