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Microsatellite markers (SSR) as a tool to assist the identification of grape (Vitis vinifera) cultivars

Author:
Xuan, H., Horny, J., Sturm, J.
Source:
Acta horticulturae 2016 no.1110 pp. 133-140
ISSN:
0567-7572
Subject:
Vitis vinifera, breeding, chromosomes, cultivars, databases, genetic markers, grapes, microsatellite repeats, mutants, plant nurseries, polymerase chain reaction, quality control
Abstract:
30 grape cultivars were genetically analysed with 17 microsatellites markers (SSRs), well scattered within 15 chromosomes. Multiplex PCR was used and consisted of 5 PCR reactions of 3-4 SSRs. The combination of 3-4 SSRs in one multiplex PCR reaction was screened out according to the fragment length and their compatibility which must present typical profiles of SSRs after PCR. As a result, all cultivars studied were separated from each other with the 17 SSR markers. Misidentified cultivar 'Sauvignonasse (Fruilano)' was detected as 'Sauvignon blanc'. The SSR-based identification method could not differentiate the mutants: 'Gutedel, Roter Klon H'; 'Gutedel, Weißer Klon We 1-6'; 'Riesling, Roter' and 'Riesling, Weißer Klon We 158'. Future extension of the fingerprinting databank with additional cultivars will generate a resource for breeding, plant nurseries, and quality control.
Agid:
5498070