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Lanostane Triterpenes from the Tibetan Medicinal Mushroom Ganoderma leucocontextum and Their Inhibitory Effects on HMG-CoA Reductase and α-Glucosidase

Wang, Kai, Bao, Li, Xiong, Weiping, Ma, Ke, Han, Junjie, Wang, Wenzhao, Yin, Wenbing, Liu, Hongwei
Journal of natural products 2015 v.78 no.8 pp. 1977-1989
Ganoderma lucidum, alpha-glucosidase, cytotoxicity, fruiting bodies, hydroxymethylglutaryl-CoA reductases, inhibitory concentration 50, medicinal fungi, paclitaxel, secondary metabolites, spectral analysis, triterpenoids, yeasts
Sixteen new lanostane triterpenes, ganoleucoins A–P (1–16), together with 10 known tripterpenes (17–26), were isolated from the cultivated fruiting bodies of Ganoderma leucocontextum, a new member of the Ganoderma lucidum complex. The structures of the new compounds were elucidated by extensive spectroscopic analysis and chemical transformation. The inhibitory effects of 1–26 on HMG-CoA reductase and α-glucosidase were tested in vitro. Compounds 1, 3, 6, 10–14, 17, 18, 23, 25, and 26 showed much stronger inhibitory activity against HMG-CoA reductase than the positive control atorvastatin. Compounds 13, 14, and 16 presented potent inhibitory activity against α-glucosidase from yeast with IC₅₀ values of 13.6, 2.5, and 5.9 μM, respectively. In addition, the cytotoxicity of 1–26 was evaluated against the K562 and PC-3 cell lines by the MTT assay. Compounds 1, 2, 6, 7, 10, 12, 16, 18, and 25 exhibited cytotoxicity against K562 cells with IC₅₀ values in the range 10–20 μM. Paclitaxel was used as the positive control with an IC₅₀ value of 0.9 μM. This is the first report of secondary metabolites from this medicinal mushroom.