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Alkaloids from Hippeastrum argentinum and Their Cholinesterase-Inhibitory Activities: An in Vitro and in Silico Study

Author:
Ortiz, Javier E., Pigni, Natalia B., Andujar, Sebastián A., Roitman, German, Suvire, Fernando D., Enriz, Ricardo D., Tapia, Alejandro, Bastida, Jaume, Feresin, Gabriela E.
Source:
Journal of natural products 2016 v.79 no.5 pp. 1241-1248
ISSN:
1520-6025
Subject:
Hippeastrum, alkaloids, bulbs, chemical elements, cholinesterase, gas chromatography-mass spectrometry, inhibitory concentration 50, molecular dynamics, molecular models, quantum mechanics
Abstract:
Two new alkaloids, 4-O-methylnangustine (1) and 7-hydroxyclivonine (2) (montanine and homolycorine types, respectively), and four known alkaloids were isolated from the bulbs of Hippeastrum argentinum, and their cholinesterase-inhibitory activities were evaluated. These compounds were identified using GC-MS, and their structures were defined by physical data analysis. Compound 2 showed weak butyrylcholinesterase (BuChE)-inhibitory activity, with a half-maximal inhibitory concentration (IC₅₀) value of 67.3 ± 0.09 μM. To better understand the experimental results, a molecular modeling study was also performed. The combination of a docking study, molecular dynamics simulations, and quantum theory of atoms in molecules calculations provides new insight into the molecular interactions of compound 2 with BuChE, which were compared to those of galantamine.
Agid:
5498505