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Hyaluronate–Death Receptor 5 Antibody Conjugates for Targeted Treatment of Liver Metastasis

Author:
Lee, Hwiwon, Hong, Beom-Ju, Lee, Jeong Ho, Yeo, Sujin, Jung, Hoe-Yune, Chung, Junho, Ahn, G-One, Hahn, Sei Kwang
Source:
Biomacromolecules 2016 v.17 no.9 pp. 3085-3093
ISSN:
1526-4602
Subject:
binding capacity, high performance liquid chromatography, hyaluronic acid, light scattering, liver, metastasis, monoclonal antibodies, neoplasm cells, neoplasms, nuclear magnetic resonance spectroscopy, receptors, survival rate, thiols
Abstract:
The liver is the most frequent site of metastasis with a 5-year survival rate of only 20–40%. In this work, hyaluronate (HA)–death receptor 5 antibody (DR5 Ab) conjugate was synthesized as a dual targeting therapeutic agent to treat liver metastasis. Dual targeting was achieved by DR5 Ab, a humanized agonistic monoclonal antibody binding to DR5 frequently overexpressed in many kinds of cancer cells, and by HA, a natural polysaccharide binding to HA receptors highly expressed in both the liver and cancer cells. Thiol end-modified HA was site-specifically conjugated to N-glycan on Fc region of oxidized DR5 Ab using a heterobifunctional linker of 3-(2-pyridyldithio)propionyl hydrazide (PDPH). The successful synthesis of HA-DR5 Ab conjugate was confirmed by ¹H NMR, purpald assay, dynamic light scattering (DLS), and high-performance liquid chromatography (HPLC). In vitro analysis of HA-DR5 Ab conjugate revealed that the conjugation of HA to DR5 Ab did not affect the binding affinity and anticancer efficacy of DR5 Ab. Remarkably, according to in vivo bioimaging study, HA-DR5 Ab conjugate appeared to be highly accumulated in the liver and dramatically effective in inhibiting the tumor growth in liver metastasis model mice.
Agid:
5506332