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Thrips-mediated impacts from transgenic rice expressing Cry1Ab on ecological fitness of non-target predator Orius tantilus (Hemiptera: Anthocoridae)

Raen, Akhtar Zunnu, DANG, Cong, WANG, Fang, PENG, Yu-fa, YE, Gong-yin
Journal of integrative agriculture 2016 v.15 no.9 pp. 2059-2069
Bacillus thuringiensis, Lepidoptera, Orius, Oryza sativa, adults, color, enzyme-linked immunosorbent assay, fecundity, females, genes, herbivores, insecticidal proteins, larvae, life history, longevity, monitoring, oviposition, parasitoids, pollen, population dynamics, predators, rearing, rice, risk, transgenic plants, trophic levels
Various rice lines have been genetically modified with genes from Bacillus thuringiensis (Bt) to continuously produce Bt insecticidal proteins against lepidopteran larvae. The Bt insecticidal protein constantly expresses in the plants to create an opportunity for non-target herbivores to acquire and convey the protein to their predators or parasitoids across trophic levels. This paper evaluates the effects of Bt rice (namely, Kemindao 1 (KMD1) and Kemindao 2 (KMD2)) expressing Cry1Ab as compared to its non-Bt control line, Xiushui 11 on non-target predator Orius tantilus (a generalist predatory anthocorid of thrips) under laboratory and field conditions. To measure several biological parameters such as total nymphal duration and fecundity of this bug, it was reared on thrips and pollens of KMD1 and KMD2 as compared to their control under laboratory conditions. By comparison with the control, Bt rice did not significantly affect main life-history characteristics (total nymphal duration, female adult longevity, oviposition period and fecundity) of this anthocorid preying on Bt rice-fed thrips along with Bt rice pollens, except that the fecundity of this predator for KMD1 was distinctly lower as compared with KMD2 or the control. Enzyme-linked immunosorbent assay (ELISA) results showed that no Cry1Ab protein was detected in this predator fed on thrips or rice pollen from Bt rice but was in Bt rice pollens. With the beat plate, plastic bag and color trap sampling methods, two-year field monitoring of O. tantilus abundance demonstrated that Bt rice had no significant detrimental effects on the population dynamics and seasonal average densities of this predatory anthocorid as compared with the control. Thus, it is suggested that growing our tested Bt rice (KMD1 and KMD2) producing Cry1Ab will pose a negligible risk to the anthocorid, O. tantilus.