PubAg

Main content area

A new ultra-high pressure liquid chromatography method for the determination of antioxidant flavonol aglycones in six Lysimachia species

Author:
Tóth, Anita, Végh, Krisztina, Alberti, Ágnes, Béni, Szabolcs, Kéry, Ágnes
Source:
Natural product research 2016 v.30 no.20 pp. 2372-2377
ISSN:
1478-6427
Subject:
2,2-diphenyl-1-picrylhydrazyl, Lysimachia clethroides, Lysimachia nummularia, acid hydrolysis, antioxidant activity, decolorization, free radical scavengers, kaempferol, liquid chromatography, methanol, myricetin, quantitative analysis, quercetin
Abstract:
UPLC-DAD method was developed and validated for the quantitative determination of free flavonol aglycones (kaempferol, quercetin and myricetin) after acidic hydrolysis in six Lysimachia species. Quantitative analyses showed that the amounts of various flavonol aglycones were significantly different in Lysimachia vulgaris , Lysimachia nummularia , Lysimachia punctata , Lysimachia christinae , Lysimachia ciliata and Lysimachia clethroides . The L. clethroides sample was found to be the richest in kaempferol (25.77 ± 1.29 μg/mg extract) and quercetin (97.67 ± 4.61 μg/mg extract), while the L. nummularia sample contained the highest amount of myricetin (20.79 ± 1.00 μg/mg extract). The antioxidant capacity of hydrolysed extracts was evaluated using in vitro DPPH • (2,2-diphenyl-1-picrylhydrazyl) and ABTS •⁺ [2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid)] decolourisation tests. The observed radical scavenging capacities of the extracts showed a relationship with the measured flavonol aglycone content and composition. The acidic treatment resulted in an increased free radical scavenging activity compared to the untreated methanol extract.
Agid:
5507857