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A Workflow to Preserve Genome-Quality Tissue Samples From Plants in Botanical Gardens and Arboreta

Gostel, Morgan R., Kelloff, Carol, Wallick, Kyle, Funk, Vicki A.
Applications in plant sciences 2016 v.4 no.9
arboreta, botanical gardens, botanists, databases, genome, genomics, herbaria, leaves, nitrogen, silica, vertebrates
Premise of the study: Internationally, gardens hold diverse living collections that can be preserved for genomic research. Work-flows have been developed for genomic tissue sampling in other taxa (e.g., vertebrates), but are inadequate for plants. We outline a workflow for tissue sampling intended for two audiences: botanists interested in genomics research and garden staff who plan to voucher living collections. Methods and Results: Standard herbarium methods are used to collect vouchers, label information and images are entered into a publicly accessible database, and leaf tissue is preserved in silica and liquid nitrogen. A five-step approach for genomic tissue sampling is presented for sampling from living collections according to current best practices. Conclusions: Collecting genome-quality samples from gardens is an economical and rapid way to make available for scientific research tissue from the diversity of plants on Earth. The Global Genome Initiative will facilitate and lead this endeavor through international partnerships.