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Development of seven novel EST-SSR markers from Cycas panzhihuaensis (Cycadaceae)

Author:
Zhang, Fangming, Su, Tao, Yang, Ying, Zhai, Yanhong, Ji, Yunheng, Chen, Shaotian
Source:
American journal of botany 2010 v.97 no.12 pp. e159
ISSN:
0002-9122
Subject:
Coniferophyta, Cycas, alleles, databases, expressed sequence tags, genetic markers, genetic variation, heterozygosity, microsatellite repeats, population genetics, population structure, researchers, China
Abstract:
Premise of the study: Cycas panzhihuaensis L. Zhou & S. Y. Yang is a vulnerable gymnosperm endemic to China, where its range represents the northernmost occupation of Cycas L. We developed EST-derived SSR markers to investigate its genetic diversity and population structure. METHODS: and Results: Based on the expressed sequence tag (EST) database for Cycas rumphii Miq., seven simple sequence repeat (SSR) markers were identified and screened in 55 individuals from seven wild populations of C. panzhihuaensis. Alleles numbered 2 to 5, and their observed heterozygosity and expected heterozygosity ranged from 0.0000 to 0.6545 and from 0.0535 to 0.6966, respectively. CONCLUSIONS: These new EST-SSR markers will enhance further studies of the population genetics of C. panzhihuaensis, allowing researchers to design reasonable conservation and management protocols.
Agid:
550922