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Characterization, antioxidant and immunomodulatory activities of polysaccharides from Prunella vulgaris Linn

Li, Chao, Huang, Qiang, Fu, Xiong, Yue, Xiu-Jie, Liu, Rui Hai, You, Li-Jun
International journal of biological macromolecules 2015 v.75 pp. 298-305
Prunella vulgaris, alternative medicine, antioxidant activity, antioxidants, cytotoxicity, functional foods, immunomodulation, interleukin-6, macrophages, mice, molecular weight, nitric oxide, polysaccharides, sodium chloride, tumor necrosis factor-alpha
Water-soluble polysaccharides from Prunella vulgaris Linn (P. vulgaris) were fractionated using DEAE-Sepharose fast-flow column to obtain several eluents of water (PV-P1), 0.1M NaCl (PV-P2) and 0.2M NaCl (PV-P3). Structural analyses showed that PV-P1 had a higher molecular weight and degree of branching as compared to PV-P2 and PV-P3. Tertiary structure analyses indicated that PV-P1, PV-P2 and PV-P3 did not have triple-helical conformation. PV-P2 and PV-P3 showed stronger antioxidant activities than PV-P1, as measured radical scavenging capacities. PV-P1 showed stronger immunomodulatory activities than PV-P2 and PV-P3 in term of stimulation of the production of pro-inflammatory cytokines, including nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in murine macrophage RAW 264.7 cells. PV-P1, PV-P2 and PV-P3 did not exhibit cytotoxicities against RAW 264.7 at the concentrations tested. These results suggest that P. vulgaris polysaccharides could be explored as potential antioxidant and immunomodulatory agents for the complementary medicine or functional foods.