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Nano-hydroxyapatite/chitosan–starch nanocomposite as a novel bone construct: Synthesis and in vitro studies

Shakir, Mohammad, Jolly, Reshma, Khan, Mohd Shoeb, Iram, Noor e, Khan, Haris M.
International journal of biological macromolecules 2015 v.80 pp. 282-292
Fourier transform infrared spectroscopy, Gram-negative bacteria, X-ray diffraction, ambient temperature, antibacterial properties, biocompatibility, bones, chitosan, coprecipitation, crystallites, cytotoxicity, fibroblasts, humans, hydroxyapatite, in vitro studies, mice, nanocomposites, scanning electron microscopy, starch, strength (mechanics), thermal stability, tissue engineering, transmission electron microscopy
A novel ternary nanocomposite system incorporating hydroxyapatite, chitosan and starch (n-HA/CS–ST) has been synthesized by co-precipitation method at room temperature, addressing the issues of biocompatibility, mechanical strength and cytotoxicity required for bone tissue engineering. The interactions, crystallite size, surface morphology and thermal stability against n-HA/CS nanocomposite have been obtained by comparing the results of FTIR, SEM, TEM, DLS, XRD and TGA/DTA. A comparative study of bioactivity and thermal stability of n-HA/CS and n-HA/CS–ST nanocomposites revealed that the incorporation of starch as templating agent enhanced these properties in n-HA/CS–ST nanocomposite. A lower swelling rate of n-HA/CS–ST relative to n-HA/CS indicates a higher mechanical strength supportive of bone tissue ingrowths. The MTT assay on murine fibroblast L929 and human osteoblasts-like MG-63 cells and in vitro bioactivity of n-HA/CS–ST matrix referred superior non-toxic nature of n-HA/CS–ST nanocomposite and greater possibility of osteointegration in vivo respectively. Furthermore n-HA/CS–ST exhibited improved antibacterial property against both Gram-positive and Gram-negative bacteria relative to n-HA/CS.