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Optimization and antioxidant activity of polysaccharides from Plantago depressa

Author:
Han, Na, Wang, Lin, Song, Zehai, Lin, Junyu, Ye, Chun, Liu, Zhihui, Yin, Jun
Source:
International journal of biological macromolecules 2016 v.93 pp. 644-654
ISSN:
0141-8130
Subject:
2,2-diphenyl-1-picrylhydrazyl, Plantago, antioxidant activity, arabinose, beta-carotene, bleaching, ethanol, free radicals, galactose, glucose, high performance liquid chromatography, models, nuclear magnetic resonance spectroscopy, polysaccharides, raw materials, rhamnose
Abstract:
Polysaccharide from the herb of Plantago depressa (PDP) was obtained through ethanol precipitation preceded by a water extraction step. The optimum extraction yield of 5.68±0.46% was obtained with the treatment of raw material in water (w/v, 1:25.34) at 80.44°C during 1.97h, 3.28 times. Under these conditions, obtained yield value was in total agreement with value predicted by the model executed by Box-Behnken design (BBD). Following analysis by IR, HPLC-UV, MS and 1H NMR, the composition of PDP was found to be l-rhamnose, galactose, arabinose, glucose and d-galacturonic acid. The maximum tolerated dose of PDP was 10g/kg. The antioxidant activity of PDP was investigated using five tests and it was found that PDP was able to scavenge hydroxyl, DPPH and ABTS radicals, besides their β-carotene bleaching inhibitory activity. In particular, in the test of β-carotene bleaching inhibition, PDP displayed higher activity than Vitamin C.
Agid:
5519566