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Optimization and antioxidant activity of polysaccharides from Plantago depressa

Han, Na, Wang, Lin, Song, Zehai, Lin, Junyu, Ye, Chun, Liu, Zhihui, Yin, Jun
International journal of biological macromolecules 2016 v.93 pp. 644-654
2,2-diphenyl-1-picrylhydrazyl, Plantago, antioxidant activity, arabinose, beta-carotene, bleaching, ethanol, free radicals, galactose, glucose, high performance liquid chromatography, models, nuclear magnetic resonance spectroscopy, polysaccharides, raw materials, rhamnose
Polysaccharide from the herb of Plantago depressa (PDP) was obtained through ethanol precipitation preceded by a water extraction step. The optimum extraction yield of 5.68±0.46% was obtained with the treatment of raw material in water (w/v, 1:25.34) at 80.44°C during 1.97h, 3.28 times. Under these conditions, obtained yield value was in total agreement with value predicted by the model executed by Box-Behnken design (BBD). Following analysis by IR, HPLC-UV, MS and 1H NMR, the composition of PDP was found to be l-rhamnose, galactose, arabinose, glucose and d-galacturonic acid. The maximum tolerated dose of PDP was 10g/kg. The antioxidant activity of PDP was investigated using five tests and it was found that PDP was able to scavenge hydroxyl, DPPH and ABTS radicals, besides their β-carotene bleaching inhibitory activity. In particular, in the test of β-carotene bleaching inhibition, PDP displayed higher activity than Vitamin C.