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Enzymatically controlled material design with casein—From defined films to localized deposition of particles
- Strube, Oliver I., Rüdiger, Arne A., Bremser, Wolfgang
- Journal of biotechnology 2015 v.201 pp. 69-74
- casein, cheesemaking, chymosin, coagulation, coatings, enzymatic reactions, hydrophilicity, micelles, rennet
- A new concept for deposition and material design of coatings from biological compounds is presented. An enzymatic reaction triggers the specific coagulation of particles on a support surface. The first examined model system is casein and is based on the natural rennet reaction as applied in the process of cheese-making. The aspartic protease chymosin is immobilized on a support surface and cleaves the hydrophilic parts of the casein micelles, inducing deposition. The concept allows for a high level of control over film characteristics and enables the formation of site-specific film structures. The variability rages from formation of casein films with several micrometers film thickness to the targeted deposition of casein micelles.