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Anti-proliferative activity of 25-hydroxyvitamin D3 in human prostate cells
- Munetsuna, Eiji, Kawanami, Rie, Nishikawa, Miyu, Ikeda, Shinnosuke, Nakabayashi, Sachie, Yasuda, Kaori, Ohta, Miho, Kamakura, Masaki, Ikushiro, Shinichi, Sakaki, Toshiyuki
- Molecular and Cellular Endocrinology 2014 v.382 pp. 960-970
- 25-hydroxycholecalciferol, DNA microarrays, bioactive properties, cell culture, cell growth, gene expression, genes, humans, messenger RNA, quantitative polymerase chain reaction, reverse transcriptase polymerase chain reaction
- 1α-Hydroxylation of 25-hydroxyvitamin D3 is believed to be essential for its biological effects. In this study, we evaluated the biological activity of 25(OH)D3 itself comparing with the effect of cell-derived 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3). First, we measured the cell-derived 1α,25(OH)2D3 level in immortalized human prostate cell (PZ-HPV-7) using [3H]-25(OH)D3. The effects of the cell-derived 1α,25(OH)2D3 on vitamin D3 24-hydroxylase (CYP24A1) mRNA level and the cell growth inhibition were significantly lower than the effects of 25(OH)D3 itself added to cell culture. 25-Hydroxyvitamin D3 1α-hydroxylase (CYP27B1) gene knockdown had no significant effects on the 25(OH)D3-dependent effects, whereas vitamin D receptor (VDR) gene knockdown resulted in a significant decrease in the 25(OH)D3-dependent effects. These results strongly suggest that 25(OH)D3 can directly bind to VDR and exerts its biological functions. DNA microarray and real-time RT–PCR analyses suggest that semaphorin 3B, cystatin E/M, and cystatin D may be involved in the antiproliferative effect of 25(OH)D3.