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Cloning, expression, and characterization of miR058 and its target PPO during the development of grapevine berry stone
- Ren, Guohui, Wang, Baoju, Zhu, Xudong, Mu, Qian, Wang, Chen, Tao, Ran, Fang, Jinggui
- Gene 2014 v.548 pp. 166-173
- Vitis, bioinformatics, cultivars, enzymes, genes, lignin, oxidation, polyphenols, pulp, quantitative polymerase chain reaction, quinones, reverse transcriptase polymerase chain reaction, seeds
- Polyphenol oxidases catalyzing the oxygen-dependent oxidation of phenols to quinones are ubiquitous among angiosperms. They are key enzymes playing a significant role during the synthesis of lignin. The inhibition of the synthesis of lignin in grapevine can cause seedless grapevine berry development. In this study, grapevine PPO (Vv-PPO) was predicted as the target gene of Vv-miR058 by bioinformatics analysis, and it was further cloned and its homologous conservation in various plants was analyzed. The expression profiles of miR058 and its target Vv-PPO were detected by qRT-PCR in peel, pulp and seeds of three grapevine cultivars and Vv-PPO was expressed in an opposite variation way with Vv-miR058 where both of them could be detected, suggesting that Vv-miR058 can play an important role by regulating the expression of Vv-PPO. In addition, the potential target gene Vv-PPO for Vv-miR058 was verified by RLM-RACE. This result would be helpful in theoretical basis for further research and seedless grapevine berry production.