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Molecular and functional characterization of the ecdysone receptor isoform-A from the cotton mirid bug, Apolygus lucorum (Meyer-Dür)

Author:
Tan, Yong-An, Xiao, Liu-Bin, Zhao, Jing, Sun, Yang, Bai, Li-Xin
Source:
Gene 2015 v.574 pp. 88-94
ISSN:
0378-1119
Subject:
Hemiptera, Northern blotting, RNA interference, adults, amino acids, antisense RNA, complementary DNA, cotton, ecdysone receptor, fat body, genes, insects, instars, messenger RNA, mortality, nymphs, open reading frames, phylogeny
Abstract:
We cloned the cDNA of the ecdysone receptor (EcR) isoform-A from the mirid bug, Apolygus lucorum (AlEcR-A). The AlEcR-A cDNA has an open reading frame of 1410bp with a conserved sequence of approximately 20 amino acids at the carboxyl-end of its A/B-specific domain. Phylogenetic analysis showed that AlEcR-A is very similar to the EcR-A genes of other Hemiptera species. AlEcR-A mRNA was detected at all developmental stages of A. lucorum with peaks correlating to ecdysteroid pulses. AlEcR-A was also expressed in all analyzed tissues with maximum expression in the epidermis and fat body. An AlEcR-A mRNA of size 1.8kb was detected in all tissues by northern blot analysis. We investigated the functions of AlEcR-A in A. lucorum growth and development using RNAi in vivo. Weights of fifth instar nymphs were significantly decreased in insects treated with AlEcR-A specific anti-sense RNA. Mortality from third instar nymphs to adults increased significantly along with a significant increase in instar duration.
Agid:
5525091