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Molecular and functional characterization of the ecdysone receptor isoform-A from the cotton mirid bug, Apolygus lucorum (Meyer-Dür)

Tan, Yong-An, Xiao, Liu-Bin, Zhao, Jing, Sun, Yang, Bai, Li-Xin
Gene 2015 v.574 pp. 88-94
Hemiptera, Northern blotting, RNA interference, adults, amino acids, antisense RNA, complementary DNA, cotton, ecdysone receptor, fat body, genes, insects, instars, messenger RNA, mortality, nymphs, open reading frames, phylogeny
We cloned the cDNA of the ecdysone receptor (EcR) isoform-A from the mirid bug, Apolygus lucorum (AlEcR-A). The AlEcR-A cDNA has an open reading frame of 1410bp with a conserved sequence of approximately 20 amino acids at the carboxyl-end of its A/B-specific domain. Phylogenetic analysis showed that AlEcR-A is very similar to the EcR-A genes of other Hemiptera species. AlEcR-A mRNA was detected at all developmental stages of A. lucorum with peaks correlating to ecdysteroid pulses. AlEcR-A was also expressed in all analyzed tissues with maximum expression in the epidermis and fat body. An AlEcR-A mRNA of size 1.8kb was detected in all tissues by northern blot analysis. We investigated the functions of AlEcR-A in A. lucorum growth and development using RNAi in vivo. Weights of fifth instar nymphs were significantly decreased in insects treated with AlEcR-A specific anti-sense RNA. Mortality from third instar nymphs to adults increased significantly along with a significant increase in instar duration.