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Complete genome sequencing and comparative analyses of broad-spectrum antimicrobial-producing Micromonospora sp. HK10

Talukdar, Madhumita, Das, Dhrubajyoti, Bora, Chiranjeeta, Bora, Tarun Chandra, Deka Boruah, Hari Prasanna, Singh, Anil Kumar
Gene 2016 v.594 no.1 pp. 97-107
Bacillus subtilis, Escherichia coli, Micromonospora, Mycobacterium abscessus, Mycobacterium smegmatis, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella, Staphylococcus aureus, anti-infective agents, antibacterial properties, bioactive compounds, humans, multigene family, nucleotide sequences, pathogens, secondary metabolites, sequence analysis
Micromonospora genus produces >700 bioactive compounds of medical relevance. In spite of its ability to produce high number of bioactive compounds, no genome sequence is available with comprehensive secondary metabolite gene clusters analysis for anti-microbial producing Micromonospora strains. Thus, here we contribute the full genome sequence of Micromonospora sp. HK10 strain, which has high antibacterial activity against several important human pathogens like, Mycobacterium abscessus, Mycobacterium smegmatis, Bacillus subtillis, Staphylococcus aureus, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella and Escherichia coli. We have generated whole genome sequence data of Micromonospora sp. HK10 strain using Illumina NexSeq 500 sequencing platform (2×150bp paired end library) and assembled it de novo. The sequencing of HK10 genome enables identification of various genetic clusters associated with known- and probably unknown- antimicrobial compounds, which can pave the way for new antimicrobial scaffolds.