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Biosensor based on nanocomposite material for pathogenic virus detection

Author:
Van Thu, Vu, Dung, Phuong Trung, Tam, Le Thi, Tam, Phuong Dinh
Source:
Colloids and Surfaces B: Biointerfaces 2014 v.115 pp. 176-181
ISSN:
0927-7765
Subject:
DNA, biosensors, carbon nanotubes, colloids, cost effectiveness, dielectric spectroscopy, electron transfer, hybridization, nanocomposites, nucleotide sequences, storage quality, storage time, viruses
Abstract:
This paper introduces a DNA biosensor based on a DNA/chitosan/multi-walled carbon nanotube nanocomposite for pathogenic virus detection. An easy, cost-effective approach to the immobilization of probe DNA sequences on the sensor surface was performed. Cyclic voltammograms were used to characterize the probe DNA sequence immobilization. Complementary sequence hybridization was examined by electrochemical impedance spectroscopy. Results revealed that the developed DNA sensor can detect a target DNA concentration as low as 0.01×10−12M. The sensitivity of the prepared sensor was 52.57kΩ/fM. The reusability and storage stability of the DNA sensor were also investigated. Results showed that the electron-transfer resistance decreased to approximately 35% after 8 weeks and to approximately 80% after 12 weeks of storage.
Agid:
5530042