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Antibody-based donor–acceptor spatial reconfiguration in decorated lanthanide-doped nanoparticle colloids for the quantification of okadaic acid biotoxin

Author:
Stipić, Filip, Burić, Petra, Jakšić, Željko, Pletikapić, Galja, Dutour Sikirić, Maja, Zgrablić, Goran, Frkanec, Leo, Lyons, Daniel M.
Source:
Colloids and Surfaces B: Biointerfaces 2015 v.135 pp. 481-489
ISSN:
0927-7765
Subject:
antibodies, bioassays, biochemical compounds, biosensors, colloids, detection limit, energy transfer, fluorescent dyes, mice, nanoparticles, okadaic acid, photoluminescence, shellfish, toxins
Abstract:
With the increasing movement away from the mouse bioassay for the detection of toxins in commercially harvested shellfish, there is a growing demand for the development of new and potentially field-deployable tests in its place. In this direction we report the development of a simple and sensitive nanoparticle-based luminescence technique for the detection of the marine biotoxin okadaic acid. Photoluminescent lanthanide nanoparticles were conjugated with fluorophore-labelled anti-okadaic acid antibodies which, upon binding to okadaic acid, gave rise to luminescence resonance energy transfer from the nanoparticle to the organic fluorophore dye deriving from a reduction in distance between the two. The intensity ratio of the fluorophore: nanoparticle emission peaks was found to correlate with okadaic acid concentration, and the sensor showed a linear response in the 0.37–3.97μM okadaic acid range with a limit of detection of 0.25μM. This work may have important implications for the development of new, cheap, and versatile biosensors for a range of biomolecules and that are sufficiently simple to be applied in the field or at point-of-care.
Agid:
5531196