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Antibody-based donor–acceptor spatial reconfiguration in decorated lanthanide-doped nanoparticle colloids for the quantification of okadaic acid biotoxin

Stipić, Filip, Burić, Petra, Jakšić, Željko, Pletikapić, Galja, Dutour Sikirić, Maja, Zgrablić, Goran, Frkanec, Leo, Lyons, Daniel M.
Colloids and Surfaces B: Biointerfaces 2015 v.135 pp. 481-489
antibodies, bioassays, biochemical compounds, biosensors, colloids, detection limit, energy transfer, fluorescent dyes, mice, nanoparticles, okadaic acid, photoluminescence, shellfish, toxins
With the increasing movement away from the mouse bioassay for the detection of toxins in commercially harvested shellfish, there is a growing demand for the development of new and potentially field-deployable tests in its place. In this direction we report the development of a simple and sensitive nanoparticle-based luminescence technique for the detection of the marine biotoxin okadaic acid. Photoluminescent lanthanide nanoparticles were conjugated with fluorophore-labelled anti-okadaic acid antibodies which, upon binding to okadaic acid, gave rise to luminescence resonance energy transfer from the nanoparticle to the organic fluorophore dye deriving from a reduction in distance between the two. The intensity ratio of the fluorophore: nanoparticle emission peaks was found to correlate with okadaic acid concentration, and the sensor showed a linear response in the 0.37–3.97μM okadaic acid range with a limit of detection of 0.25μM. This work may have important implications for the development of new, cheap, and versatile biosensors for a range of biomolecules and that are sufficiently simple to be applied in the field or at point-of-care.