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Biocontrol and plant growth promoting activities of a Streptomyces corchorusii strain UCR3-16 and preparation of powder formulation for application as biofertilizer agents for rice plant

Author:
Tamreihao, K., Ningthoujam, Debananda S., Nimaichand, Salam, Singh, Elangbam Shanta, Reena, Pascal, Singh, Salam Herojeet, Nongthomba, Upendra
Source:
Microbiological research 2016 v.192 pp. 260-270
ISSN:
0944-5013
Subject:
1-aminocyclopropane-1-carboxylate deaminase, Oryza sativa, Streptomyces corchorusii, ammonia, antifungal properties, beta-glucanase, biofertilizers, biological control, blight, carboxylic ester hydrolases, cell walls, chitinase, corn starch, field experimentation, fungi, grain yield, indole acetic acid, leaves, pathogens, phosphates, plant growth, rice, roots, seedlings, seeds, shoots, siderophores, soil, storage time, talc, vigor, volatile compounds
Abstract:
Streptomyces corchorusii strain UCR3-16, obtained from rice rhizospheric soils showed antifungal activities against 6 major rice fungal pathogens by diffusible and volatile compounds production. The strain was found positive for production of fungal cell wall degrading enzymes such as chitinase, β-1,3-glucanase, β-1,4-glucanase, lipase and protease.The strain was also positive for plant growth promoting traits. It produced up to 30.5μg/ml of IAA and solubilized a significant amount of inorganic phosphate (up to 102μg/ml). It also produced 69% siderophore units. The strain also produced ammonia and gave positive result for ACC deaminase activity.Highest vigor index of inoculated seedlings was observed when rice seeds were treated with cell suspension of UCR3-16 corresponding to 4.5×10⁸cfu/ml. Bioinoculant-treated seeds also showed similar results under pathogen challenged conditions. In pot trial experiments, UCR3-16-treated rice plants showed significantly increased growth and grain yield production. Powder formulation of the strain was developed using talcum and corn starch as carriers and the shelf-lives were monitored. Talcum formulation showed higher cell-count than corn starch even after 6 months of storage, and optimum condition for storage of the powder formulation were found to be at 4°C. Pot trial experiments using talcum powder formulation also showed significant positive effects on growth of rice plants. Field trial using talcum powder formulation also exhibited significant enhancement in shoot length and weight of shoot and root, and total grain yield and weight of grains in rice plants. Talcum formulation also significantly reduced the sheath blight disease in rice leaves.
Agid:
5532304