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A simple and sensitive immunoassay for the determination of human chorionic gonadotropin by graphene-based chemiluminescence resonance energy transfer

Lei, Jiuqian, Jing, Tao, Zhou, Tingting, Zhou, Yusun, Wu, Wei, Mei, Surong, Zhou, Yikai
Biosensors & bioelectronics 2014 v.54 pp. 72-77
antibodies, antigen-antibody complex, antigens, biosensors, blood serum, chemiluminescence, colorimetry, detection limit, energy transfer, enzyme-linked immunosorbent assay, graphene, human chorionic gonadotropin, magnetic separation, nanoparticles, peroxidase, saliva
In this study, we report a strategy of chemiluminescence resonance energy transfer (CRET) using graphene as an efficient long-range energy acceptor. Magnetic nanoparticles were also used in CRET for simple magnetic separation and immobilization of horseradish peroxidase (HRP)-labeled anti-HCG antibody. In the design of CRET system, the sandwich-type immunocomplex was formed between human chorionic gonadotropin (HCG, antigen) and two different antibodies bridged the magnetic nanoparticles and graphene (acceptors), which led to the occurrence of CRET from chemiluminescence light source to graphene. After optimizing the experimental conditions, the quenching of chemiluminescence signal depended linearly on the concentration of HCG in the range of 0.1mIUmL−1–10mIUmL−1 and the detection limit was 0.06mIUmL−1. The proposed method was successfully applied for the determination of HCG levels in saliva and serum samples, and the results were in good agreement with the plate ELISA with colorimetric detection. It could also be developed for detection of other antigen–antibody immune complexes by using the corresponding antigens and respective antibodies.