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A novel colorimetric triple-helix molecular switch aptasensor for ultrasensitive detection of tetracycline

Ramezani, Mohammad, Mohammad Danesh, Noor, Lavaee, Parirokh, Abnous, Khalil, Mohammad Taghdisi, Seyed
Biosensors & bioelectronics 2015 v.70 pp. 181-187
antibiotic residues, biosensors, blood serum, color, colorimetry, detection limit, foods, nanogold, oligonucleotides, signal transduction, tetracycline
Detection methods of antibiotic residues in blood serum and animal derived foods are of great interest. In this study a colorimetric aptasensor was designed for sensitive, selective and fast detection of tetracycline based on triple-helix molecular switch (THMS) and gold nanoparticles (AuNPs). As a biosensor, THMS shows distinct advantages including high stability, sensitivity and preserving the selectivity and affinity of the original aptamer. In the absence of tetracycline, THMS is stable, leading to the aggregation of AuNPs by salt and an obvious color change from red to blue. In the presence of tetracycline, aptamer binds to its target, signal transduction probe (STP) leaves the THMS and adsorbs on the surface of AuNPs. So the well-dispersed AuNPs remain stable against salt-induced aggregation with a red color. The presented aptasensor showed high selectivity toward tetracyclines with a limit of detection as low as 266pM for tetracycline. The designed aptasensor was successfully applied to detect tetracycline in serum and milk.