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Sensitive detection of Campylobacter jejuni using nanoparticles enhanced QCM sensor

Masdor, Noor Azlina, Altintas, Zeynep, Tothill, Ibtisam E.
Biosensors & bioelectronics 2016 v.78 pp. 328-336
Campylobacter jejuni, Escherichia coli, Listeria monocytogenes, Salmonella Typhimurium, antibody detection, cross reaction, detection limit, food pathogens, immunosensors, mice, monoclonal antibodies, nanogold, nanoparticles, polyclonal antibodies, quartz, rabbits
A quartz crystal microbalance (QCM) sensor platform was used to develop an immunosensor for the detection of food pathogen Campylobacter jejuni. Rabbit polyclonal antibodies and commercially available mouse monoclonal antibodies against C. jejuni were investigated to construct direct, sandwich and gold-nanoparticles (AuNPs) amplified sandwich assays. The performance of the QCM immunosensor developed using sandwich assay by utilising the rabbit polyclonal antibody as the capture antibody and conjugated to AuNPs as the detection antibody gave the highest sensitivity. This sensor achieved a limit of detection (LOD) of 150 colony forming unit (CFU)mL⁻¹ of C. jejuni in solution. The QCM sensor showed excellent sensitivity and specificity for Campylobacter detection with low cross reactivity for other foodborne pathogens such as Salmonella Typhimurium, (7%) Listeria monocytogenes (3%) and Escherichia coli (0%). The development of this biosensor would help in the sensitive detection of Campylobacter which can result in reducing pre-enrichment steps; hence, reducing assay time. This work demonstrates the potential of this technology for the development of a rapid and sensitive detection method for C. jejuni.