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Reusable DNA-functionalized-graphene for ultrasensitive mercury (II) detection and removal
- Liu, Yanchen, Wang, Xiangqing, Wu, Hui
- Biosensors & bioelectronics 2017 v.87 pp. 129-135
- aqueous solutions, biosensors, detection limit, fluorescence, graphene, heavy metals, lakes, mercury, oligonucleotides, surface area, thymine, toxicity, transfer DNA
- Mercury is a bioaccumulative and highly toxic heavy metal. Thus, the removal and detection of Hg²⁺ from the environment is a major challenge. This paper reports a novel bio-nanomaterial for the simultaneous determination and removal of Hg²⁺ with the use of rGO-Fe3O4 functionalized with Hg²⁺-specific thymine oligonucleotide (T-DNA). T-DNA interacts with Hg²⁺ and changes from having a random coil into a hairpin structure, thereby increasing the fluorescence of SYBR Green I. Such fluorescence turn-on process allows the detection of Hg²⁺ in the concentration range of 1–20ng/mL, with a detection limit of 0.82ng/mL. Removal is achieved by exploiting the T-Hg²⁺-T base pairs and the large surface area of graphene; these bio-nanocomposites exhibit excellent removal efficiency (over 80%) and rapid separation from the aqueous solution. Moreover, bio-nanomaterials can be regenerated after a simple treatment. The proposed method also demonstrates the evident practicability of the simultaneous detection and removal of Hg²⁺ in lake water samples.