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Biowire platform for maturation of human pluripotent stem cell-derived cardiomyocytes

Author:
Sun, Xuetao, Nunes, Sara S.
Source:
Methods 2016 v.101 pp. 21-26
ISSN:
1046-2023
Subject:
adults, biomimetics, cardiomyocytes, cell culture, electrical treatment, embryogenesis, extracellular matrix, humans, medicine, stem cells, tissue engineering
Abstract:
Human pluripotent stem cells (hPSCs)-derived cardiomyocytes (hPSC-CMs) represent a potential indefinite cell supply for cardiac tissue engineering and possibly regenerative medicine applications. However, these cells are immature compared with adult ventricular cardiomyocytes. In order to overcome this limitation, an engineered platform, called biowire, was devised to provide cultured cardiomyocytes important biomimetic cues present during embryo development, such as three-dimensional cell culture, extracellular matrix composition, soluble factors and pacing through electrical stimulation, to induce the maturation of hPSC-CMs in vitro.
Agid:
5538445