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Establishing a synthetic pathway for high-level production of 3-hydroxypropionic acid in Saccharomyces cerevisiae via β-alanine

Author:
Borodina, Irina, Kildegaard, Kanchana R., Jensen, Niels B., Blicher, Thomas H., Maury, Jérôme, Sherstyk, Svetlana, Schneider, Konstantin, Lamosa, Pedro, Herrgård, Markus J., Rosenstand, Inger, Öberg, Fredrik, Forster, Jochen, Nielsen, Jens
Source:
Metabolic engineering 2015 v.27 pp. 57-64
ISSN:
1096-7176
Subject:
Bacillus cereus, Saccharomyces cerevisiae, batch fermentation, biosynthesis, carbon dioxide, feedstocks, fossils, glucose, greenhouse gas emissions, models, pH, plastics
Abstract:
Microbial fermentation of renewable feedstocks into plastic monomers can decrease our fossil dependence and reduce global CO2 emissions. 3-Hydroxypropionic acid (3HP) is a potential chemical building block for sustainable production of superabsorbent polymers and acrylic plastics. With the objective of developing Saccharomyces cerevisiae as an efficient cell factory for high-level production of 3HP, we identified the β-alanine biosynthetic route as the most economically attractive according to the metabolic modeling. We engineered and optimized a synthetic pathway for de novo biosynthesis of β-alanine and its subsequent conversion into 3HP using a novel β-alanine-pyruvate aminotransferase discovered in Bacillus cereus. The final strain produced 3HP at a titer of 13.7±0.3gL−1 with a 0.14±0.0C-molC-mol−1 yield on glucose in 80h in controlled fed-batch fermentation in mineral medium at pH 5, and this work therefore lays the basis for developing a process for biological 3HP production.
Agid:
5539244