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Lyophilized Rhodotorula yeast as all-in-one redox biocatalyst: Access to enantiopure building blocks by simple chemoenzymatic one-pot procedures B Enzymatic

Author:
Aguirre-Pranzoni, Celeste, Bisogno, Fabricio R., Orden, Alejandro A., Kurina-Sanz, Marcela
Source:
Journal of molecular catalysis 2015 v.114 pp. 19-24
ISSN:
1381-1177
Subject:
Rhodotorula, biocatalysts, catalytic activity, enantiomers, epoxides, freeze drying, glycols, pH, solvents, stereochemistry, yeasts
Abstract:
Rhodotorula sp. LSL, isolated from a local landfarming was able to catalyze the reduction of prochiral arylketones into sec-alcohols with excellent Prelog stereoselectivity (ee>99%). The use of resting and lyophilized cells was optimized accessing to an easy-to-use whole cell biocatalyst that efficiently works even under non-sterile conditions, without the addition of external cofactors and using plain water as solvent. The catalyst resistance in alkaline media enabled (chemo)enzymatic one-pot procedures at high pH values. So a simple and efficient methodology was applied to prepare alternatively enantiopure β-halohydrins, terminal diols and epoxides from aromatic α-haloketones.
Agid:
5542010