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Separation of phenolic acids and flavonoids from Trollius chinensis Bunge by high speed counter-current chromatography

Author:
Qin, Yanhua, Liang, Yizeng, Ren, Dabing, Qiu, Ximin, Li, Xi
Source:
Journal of Chromatography B 2015 v.1001 pp. 82-89
ISSN:
1570-0232
Subject:
Trollius chinensis, chemical structure, countercurrent chromatography, electrospray ionization mass spectrometry, ethanol, ethyl acetate, flavonoids, hexane, methanol, screening, solvents, vanillic acid
Abstract:
In this work, eleven compounds were successfully separated from Trollius chinensis Bunge by using a two-step high-speed counter-current chromatography (HSCCC) method. NRTL-SAC (nonrandom two-liquid segment activity coefficient) method, a newly developed solvent system selection strategy, was applied to screening the suitable biphasic liquid systems. Hexane/ethyl acetate/ethanol/water (3:7:3:7, v/v) solvent system was used in the first step, while the hexane/ethyl acetate/methanol/water (1:2:1:2, 1:4:1:4, 1:9:1:9, v/v) systems were employed in the second step. The chemical structures of the separated compounds were identified by UV, high resolution ESI-MS and MS/MS data. The separated compounds are 3,4-dihydroxyphenylethanol (1), vanillic acid (2), orientin (3), vitexin (4), veratric acid (5), 2″-O-(3‴, 4‴-dimethoxybenzoyl) orientin (6), 2″-O-feruloylorientin (7), 2″-O-feruloylvitexin (8), 2″-O-(2‴-methylbutyryl) vitexin (9), 2″-O-(2‴-methylbutyryl) isoswertiajaponin (10), 2″-O-(2‴-methylbutyryl) isoswertisin (11). The results demonstrate that HSCCC is a powerful tool for the separation of compounds from extremely complex samples.
Agid:
5545004